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sdf1a  (R&D Systems)


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    Structured Review

    R&D Systems sdf1a
    Sdf1a, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+cxcl12+sdf1a/Recombinant+Human%2FRhesus+Macaque%2FFeline+CXCL12%2FSDF-1+alpha/pm39729736-195-29-40
    Average 95 stars, based on 139 article reviews
    sdf1a - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Single-Cell Atlas of Lineage States, Tumor Microenvironment, and Subtype-Specific Expression Programs in Gastric Cancer
    Article Snippet: Gastric cancer cells were resuspended in 600 μL of migration buffer (0.5% BSA-RPMI 1640) and seeded into the bottom chamber of 24-well transwell plate (catalog no. 3421; Corning) and allowed to settle for 4 to 6 hours. .. Recombinant human CXCL12/ SDF1a (350-NS-010-CF; R&D Systems) resuspended in 600 μL migration buffer (final concentration of 200 ng/mL) or migration buffer alone were used as positive and negative controls, respectively. ..

    Article Title: Single-Cell Atlas of Lineage States, Tumor Microenvironment, and Subtype-Specific Expression Programs in Gastric Cancer
    Article Snippet: Gastric cancer cells were resuspended in 600 μL of migration buffer (0.5% BSA-RPMI 1640) and seeded into the bottom chamber of 24-well transwell plate (catalog no. 3421; Corning) and allowed to settle for 4 to 6 hours. .. Recombinant human CXCL12/SDF1a (350-NS-010-CF; R&D Systems) resuspended in 600 μL migration buffer (final concentration of 200 ng/mL) or migration buffer alone were used as positive and negative controls, respectively. ..

    Migration:

    Article Title: Single-Cell Atlas of Lineage States, Tumor Microenvironment, and Subtype-Specific Expression Programs in Gastric Cancer
    Article Snippet: Gastric cancer cells were resuspended in 600 μL of migration buffer (0.5% BSA-RPMI 1640) and seeded into the bottom chamber of 24-well transwell plate (catalog no. 3421; Corning) and allowed to settle for 4 to 6 hours. .. Recombinant human CXCL12/ SDF1a (350-NS-010-CF; R&D Systems) resuspended in 600 μL migration buffer (final concentration of 200 ng/mL) or migration buffer alone were used as positive and negative controls, respectively. ..

    Article Title: Single-Cell Atlas of Lineage States, Tumor Microenvironment, and Subtype-Specific Expression Programs in Gastric Cancer
    Article Snippet: Gastric cancer cells were resuspended in 600 μL of migration buffer (0.5% BSA-RPMI 1640) and seeded into the bottom chamber of 24-well transwell plate (catalog no. 3421; Corning) and allowed to settle for 4 to 6 hours. .. Recombinant human CXCL12/SDF1a (350-NS-010-CF; R&D Systems) resuspended in 600 μL migration buffer (final concentration of 200 ng/mL) or migration buffer alone were used as positive and negative controls, respectively. ..

    Concentration Assay:

    Article Title: Single-Cell Atlas of Lineage States, Tumor Microenvironment, and Subtype-Specific Expression Programs in Gastric Cancer
    Article Snippet: Gastric cancer cells were resuspended in 600 μL of migration buffer (0.5% BSA-RPMI 1640) and seeded into the bottom chamber of 24-well transwell plate (catalog no. 3421; Corning) and allowed to settle for 4 to 6 hours. .. Recombinant human CXCL12/ SDF1a (350-NS-010-CF; R&D Systems) resuspended in 600 μL migration buffer (final concentration of 200 ng/mL) or migration buffer alone were used as positive and negative controls, respectively. ..

    Article Title: Single-Cell Atlas of Lineage States, Tumor Microenvironment, and Subtype-Specific Expression Programs in Gastric Cancer
    Article Snippet: Gastric cancer cells were resuspended in 600 μL of migration buffer (0.5% BSA-RPMI 1640) and seeded into the bottom chamber of 24-well transwell plate (catalog no. 3421; Corning) and allowed to settle for 4 to 6 hours. .. Recombinant human CXCL12/SDF1a (350-NS-010-CF; R&D Systems) resuspended in 600 μL migration buffer (final concentration of 200 ng/mL) or migration buffer alone were used as positive and negative controls, respectively. ..



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    FIG. 2. Pre-incubation of three-dimensional heparin– collagen scaffolds with stromal cell–derived factor 1 alpha <t>(SDF1a)</t> markedly enhances their population by cells upon implantation. Hematoxylin and eosin stain- ing of sections of heparin– collagen scaffolds incubated without (A, C) and with (B, D) SDF1a before implantation. Scaffolds were collected 1 week (A, B) or 5 weeks (C, D) post-implantation. Original magnification: 100. Experi- ment was performed twice in duplicate.
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    Image Search Results


    FIG. 2. Pre-incubation of three-dimensional heparin– collagen scaffolds with stromal cell–derived factor 1 alpha (SDF1a) markedly enhances their population by cells upon implantation. Hematoxylin and eosin stain- ing of sections of heparin– collagen scaffolds incubated without (A, C) and with (B, D) SDF1a before implantation. Scaffolds were collected 1 week (A, B) or 5 weeks (C, D) post-implantation. Original magnification: 100. Experi- ment was performed twice in duplicate.

    Journal: Tissue engineering. Part A

    Article Title: In vivo recruitment of hematopoietic cells using stromal cell-derived factor 1 alpha-loaded heparinized three-dimensional collagen scaffolds.

    doi: 10.1089/ten.tea.2008.0348

    Figure Lengend Snippet: FIG. 2. Pre-incubation of three-dimensional heparin– collagen scaffolds with stromal cell–derived factor 1 alpha (SDF1a) markedly enhances their population by cells upon implantation. Hematoxylin and eosin stain- ing of sections of heparin– collagen scaffolds incubated without (A, C) and with (B, D) SDF1a before implantation. Scaffolds were collected 1 week (A, B) or 5 weeks (C, D) post-implantation. Original magnification: 100. Experi- ment was performed twice in duplicate.

    Article Snippet: Recombinant human SDF1a was from R&D Systems (Minneapolis, MN).

    Techniques: Incubation, Derivative Assay, H&E Stain

    FIG. 3. The ability of stromal cell–derived factor 1 alpha (SDF1a) to form a gradient is essential to stimulate cell re- cruitment. Hematoxylin and eosin staining of sections of non-heparinized (A–C) and heparinized (D–F) collagen scaffolds incubated without (A, D) or with (B, E) SDF1a or subjected to a SDF1a washout (C, F) before implantation. All scaffolds were collected 5 weeks after implantation. Original magnification: 200. Experiment was performed twice in duplicate.

    Journal: Tissue engineering. Part A

    Article Title: In vivo recruitment of hematopoietic cells using stromal cell-derived factor 1 alpha-loaded heparinized three-dimensional collagen scaffolds.

    doi: 10.1089/ten.tea.2008.0348

    Figure Lengend Snippet: FIG. 3. The ability of stromal cell–derived factor 1 alpha (SDF1a) to form a gradient is essential to stimulate cell re- cruitment. Hematoxylin and eosin staining of sections of non-heparinized (A–C) and heparinized (D–F) collagen scaffolds incubated without (A, D) or with (B, E) SDF1a or subjected to a SDF1a washout (C, F) before implantation. All scaffolds were collected 5 weeks after implantation. Original magnification: 200. Experiment was performed twice in duplicate.

    Article Snippet: Recombinant human SDF1a was from R&D Systems (Minneapolis, MN).

    Techniques: Derivative Assay, Staining, Incubation

    FIG. 4. Several cell types populate heparin–collagen scaffolds with stromal cell– derived factor 1 alpha (SDF1a) are upon implantation. Heparin–collagen scaffolds were pre-incubated with SDF1a before implantation. After 5 weeks, scaffolds were collected, and sections were stained for CD11b (A), CD11c (B), or CD31 (D). The section in panel (C) was triple stained for CD150, CD41, and CD48 to detect hematopoietic stem cells. Sections were stained for the various cell markers, as described in Materials and Methods, and counterstained with hematoxylin. Original magnification: 400 (A, B); 630 (C, D). Experiment was performed twice in duplicate. Color images available online at www.liebertonline .com=ten.

    Journal: Tissue engineering. Part A

    Article Title: In vivo recruitment of hematopoietic cells using stromal cell-derived factor 1 alpha-loaded heparinized three-dimensional collagen scaffolds.

    doi: 10.1089/ten.tea.2008.0348

    Figure Lengend Snippet: FIG. 4. Several cell types populate heparin–collagen scaffolds with stromal cell– derived factor 1 alpha (SDF1a) are upon implantation. Heparin–collagen scaffolds were pre-incubated with SDF1a before implantation. After 5 weeks, scaffolds were collected, and sections were stained for CD11b (A), CD11c (B), or CD31 (D). The section in panel (C) was triple stained for CD150, CD41, and CD48 to detect hematopoietic stem cells. Sections were stained for the various cell markers, as described in Materials and Methods, and counterstained with hematoxylin. Original magnification: 400 (A, B); 630 (C, D). Experiment was performed twice in duplicate. Color images available online at www.liebertonline .com=ten.

    Article Snippet: Recombinant human SDF1a was from R&D Systems (Minneapolis, MN).

    Techniques: Derivative Assay, Incubation, Staining